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primary human alveolar type ii epithelial cells  (Procell Inc)

 
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    Procell Inc primary human alveolar type ii epithelial cells
    Primary Human Alveolar Type Ii Epithelial Cells, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+human+alveolar+epithelial+cells/pm41265642-213-0-13?v=Procell+Inc
    Average 86 stars, based on 1 article reviews
    primary human alveolar type ii epithelial cells - by Bioz Stars, 2026-07
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    Image Search Results


    MiR‐24 overexpression alleviated cyclic stretch‐induced injury in alveolar epithelial cells in vitro. (A) RT‐qPCR analysis was used to detect the miR‐24 expression in human alveolar epithelial cells treated with CS and transfected with miR‐24 mimic. (B) CCK‐8 assays were performed to detect the viability of alveolar epithelial cells in each group. (C) RT‐qPCR and (D) western blot were conducted to determine the mRNA and protein expression of proinflammatory cytokines (TNF‐α, IL‐6, and IL‐1β) in alveolar epithelial cells. (E) Intracellular ROS levels, (F) MDA, and (G) SOD levels in each group. (H) Western blot analysis was used to detect the cellular levels of apoptotic proteins (Bcl‐2, Bax, cleaved caspase‐3) in each group. (I) Flow cytometry was performed to detect the cell apoptotic rate. (J) JC‐1 staining examined the MMP of alveolar epithelial cells after CS exposure and miR‐24 overexpression (scale bar = 50 um). (K) The ATP level in alveolar epithelial cells after indicated treatment. *** p < 0.001.

    Journal: Pulmonary Circulation

    Article Title: MiR‐24 Attenuates Oxidative Stress and Mitochondrial Apoptosis in Ventilator‐Induced Lung Injury by Targeting Bcl‐2‐related Ovarian Killer

    doi: 10.1002/pul2.70171

    Figure Lengend Snippet: MiR‐24 overexpression alleviated cyclic stretch‐induced injury in alveolar epithelial cells in vitro. (A) RT‐qPCR analysis was used to detect the miR‐24 expression in human alveolar epithelial cells treated with CS and transfected with miR‐24 mimic. (B) CCK‐8 assays were performed to detect the viability of alveolar epithelial cells in each group. (C) RT‐qPCR and (D) western blot were conducted to determine the mRNA and protein expression of proinflammatory cytokines (TNF‐α, IL‐6, and IL‐1β) in alveolar epithelial cells. (E) Intracellular ROS levels, (F) MDA, and (G) SOD levels in each group. (H) Western blot analysis was used to detect the cellular levels of apoptotic proteins (Bcl‐2, Bax, cleaved caspase‐3) in each group. (I) Flow cytometry was performed to detect the cell apoptotic rate. (J) JC‐1 staining examined the MMP of alveolar epithelial cells after CS exposure and miR‐24 overexpression (scale bar = 50 um). (K) The ATP level in alveolar epithelial cells after indicated treatment. *** p < 0.001.

    Article Snippet: Primary human alveolar epithelial cells (Passage 2) were sourced from Procell Life Science & Technology Co. Ltd. (Wuhan, China).

    Techniques: Over Expression, In Vitro, Quantitative RT-PCR, Expressing, Transfection, CCK-8 Assay, Western Blot, Flow Cytometry, Staining

    BOK is directly targeted by miR‐24. (A) RT‐qPCR analysis detected the levels of the top 5 targets potentially binding to miR‐24 via the miRDB database in alveolar epithelial cells with or without CS exposure. (B) The binding sequences between miR‐24 and BOK. (C) Luciferase reporter assays evaluated miR‐24 and BOK binding. (D) RNA‐pull‐down assays evaluated the interaction between BOK and miR‐24 in alveolar epithelial cells. (E) IHC and (F) western blot analyzes revealed protein levels of BOK in rat lung tissues in each group. (G) RT‐qPCR and western blot analyzes examined BOK levels in alveolar epithelial cells. (H) RT‐qPCR demonstrated the levels of BOK in the peripheral blood of healthy controls and ARDS patients. (I) Pearson correlation analysis signified the expression relation between BOK and miR‐24 in the peripheral blood of ARDS patients. *** p < 0.001.

    Journal: Pulmonary Circulation

    Article Title: MiR‐24 Attenuates Oxidative Stress and Mitochondrial Apoptosis in Ventilator‐Induced Lung Injury by Targeting Bcl‐2‐related Ovarian Killer

    doi: 10.1002/pul2.70171

    Figure Lengend Snippet: BOK is directly targeted by miR‐24. (A) RT‐qPCR analysis detected the levels of the top 5 targets potentially binding to miR‐24 via the miRDB database in alveolar epithelial cells with or without CS exposure. (B) The binding sequences between miR‐24 and BOK. (C) Luciferase reporter assays evaluated miR‐24 and BOK binding. (D) RNA‐pull‐down assays evaluated the interaction between BOK and miR‐24 in alveolar epithelial cells. (E) IHC and (F) western blot analyzes revealed protein levels of BOK in rat lung tissues in each group. (G) RT‐qPCR and western blot analyzes examined BOK levels in alveolar epithelial cells. (H) RT‐qPCR demonstrated the levels of BOK in the peripheral blood of healthy controls and ARDS patients. (I) Pearson correlation analysis signified the expression relation between BOK and miR‐24 in the peripheral blood of ARDS patients. *** p < 0.001.

    Article Snippet: Primary human alveolar epithelial cells (Passage 2) were sourced from Procell Life Science & Technology Co. Ltd. (Wuhan, China).

    Techniques: Quantitative RT-PCR, Binding Assay, Luciferase, Western Blot, Expressing

    MiR‐24 protected the alveolar epithelial cells from CS‐induced injury by regulating BOK. (A) RT‐qPCR detected the expression of BOK in CS‐treated alveolar epithelial cells after indicated transfection. (B) CCK‐8 assays revealed the viability of alveolar epithelial cells. (C) RT‐qPCR and (D) western blot analyzes evaluated proinflammatory cytokines levels in CS‐exposed cells. (E) Intracellular ROS levels, (F) MDA and (G) SOD levels in each group. (H) Western blot was used to measure the protein levels of apoptosis‐related proteins in each group. (I) Flow cytometry determined the apoptotic rate of CS‐treated cells in each group. (J) JC‐1 staining of CS‐exposed cells in each group. (K) The ATP levels in CS‐treated cells in each group. *** p < 0.001.

    Journal: Pulmonary Circulation

    Article Title: MiR‐24 Attenuates Oxidative Stress and Mitochondrial Apoptosis in Ventilator‐Induced Lung Injury by Targeting Bcl‐2‐related Ovarian Killer

    doi: 10.1002/pul2.70171

    Figure Lengend Snippet: MiR‐24 protected the alveolar epithelial cells from CS‐induced injury by regulating BOK. (A) RT‐qPCR detected the expression of BOK in CS‐treated alveolar epithelial cells after indicated transfection. (B) CCK‐8 assays revealed the viability of alveolar epithelial cells. (C) RT‐qPCR and (D) western blot analyzes evaluated proinflammatory cytokines levels in CS‐exposed cells. (E) Intracellular ROS levels, (F) MDA and (G) SOD levels in each group. (H) Western blot was used to measure the protein levels of apoptosis‐related proteins in each group. (I) Flow cytometry determined the apoptotic rate of CS‐treated cells in each group. (J) JC‐1 staining of CS‐exposed cells in each group. (K) The ATP levels in CS‐treated cells in each group. *** p < 0.001.

    Article Snippet: Primary human alveolar epithelial cells (Passage 2) were sourced from Procell Life Science & Technology Co. Ltd. (Wuhan, China).

    Techniques: Quantitative RT-PCR, Expressing, Transfection, CCK-8 Assay, Western Blot, Flow Cytometry, Staining